This enzyme is lyophilized from a solution containing buffer salts and a stabilizer. The product may be reconsituted at 1 mg/mL in water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/412/974/g7646dat.pdf
G7646
β-Glucuronidase from Escherichia coli
Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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5000 UNITS
$55.90
25000 UNITS
$138.00
100000 UNITS
$479.00
500000 UNITS
$1,630.00
About This Item
$55.90
Check Cart for Availability
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type
Type VII-A
Quality Level
form
lyophilized powder
specific activity
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
mol wt
290 kDa
composition
Protein, ~25% biuret
storage temp.
−20°C
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Related Categories
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Quality
Contains buffer salts and stabilizer.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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It is recommended to resuspend the product in which buffer?
1 answer-
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Is BSA or other protein components included in the overall percentage listed on the CoA for the product G7646, which is β-Glucuronidase from Escherichia coli (CAS 9001-45-0)?
1 answer-
No. BSA or other protein sources are added to the product except Potassium Phosphate and Sucrose. Although this is a fermentation product, there may be other protein residues in it in a negligible amount. The product is tested by activity, residual protein contaminants are not tested. The protein percentage is the total protein (tested by Biuret method).
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