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G6672

Sigma-Aldrich

Anti-GGH (167-180) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-γ-Glutamyl hydrolase precursor, Anti-GH

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~36 kDa

species reactivity

human

technique(s)

western blot: 1:500-1:2,000

UniProt accession no.

Q92820

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GGH(8836)

General description

γ-Glutamyl hydrolase is a lysosomal and secreted glycoprotein that is abundantly expressed in liver and kidney. It may be used as a target for inhibition to enhance the therapeutic effect of antifolates and fluorouracil.

Immunogen

synthetic peptide corresponding to amino acids 167-180 of human GGH

Application

Anti-GGH antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:500-1:2000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

GGH (γ-glutamyl hydrolase) encodes an enzyme that hydrolyzes the γ-glutamyl sidechains of antifolate and folate polyglutamates. Removal of γ-glutamyl residues from pteroylpoly-γ-glutamate yields pteroyl-α-glutamate (folic acid) and free glutamate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
069K2705

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J Galivan et al.
Seminars in oncology, 26(2 Suppl 6), 33-37 (1999-12-22)
Glutamyl hydrolase cleaves the poly-gamma-glutamate chain folate and antifolate poly-gamma-glutamates. Its cellular location is lysosomal with large amounts of the enzyme constitutively secreted. The highest levels of glutamyl hydrolase mRNA in humans is found in the liver and kidney. Baculovirus-expressed
R Yao et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(19), 10134-10138 (1996-09-17)
A cDNA encoding human gamma-glutamyl hydrolase has been identified by searching an expressed sequence tag data base and using rat gamma-glutamyl hydrolase cDNA as the query sequence. The cDNA encodes a 318-amino acid protein of Mr 35,960. The deduced amino
J Galivan et al.
Pharmacology & therapeutics, 85(3), 207-215 (2000-03-31)
gamma-Glutamyl hydrolase (GH, EC 3.4.19.9) is a lysosomal and secreted glycoprotein that hydrolyzes the gamma-glutamyl tail of antifolate and folate polyglutamates. Tumor cells that have high levels of GH are inherently resistant to classical antifolates, and further resistance can be

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