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G5796

Sigma-Aldrich

Monoclonal Anti-Gemin 8 antibody produced in mouse

~1 mg/mL, clone 1F8, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Gem associated protein 8

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1F8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

rat, mouse, human, canine

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using HeLa nuclear cell extract

isotype

IgG2a

UniProt accession no.

Q9NWZ8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GEMIN8(54960)
mouse ... Gemin8(237221)
rat ... Gemin8(363462)

Immunogen

Gemin 8 recombinant human protein.

Application

Monoclonal Anti-Gemin 8 antibody produced in mouse is suitable for immunocytochemistry, immunoprecipitation and indirect ELISA. It is also suitable for western blot analysis at a working concentration of 1-2μg/mL using HeLa nuclear cell extract.

Biochem/physiol Actions

GEMIN8 is integral for the proper structural organization of the SMN complex. It also forms a heteromeric complex with Gemin6, Gemin7 and Unrip and mediates recruitment of Sm protein to the snRNP pathway.

Target description

GEMIN8 encodes a protein that forms a part of the SMN (Survival of Motor Neurons) complex. The complex is involved in spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing and transcription in the nucleus. SMN forms a multi-protein complex with Gemin 2-7 proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
100M4814
058K4824

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Claudia Carissimi et al.
The Journal of biological chemistry, 281(12), 8126-8134 (2006-01-26)
The survival motor neuron (SMN) protein is the product of the spinal muscular atrophy disease gene. SMN and Gemin2-7 proteins form a large macromolecular complex that localizes in the cytoplasm as well as in the nucleoplasm and in nuclear Gems.
Claudia Carissimi et al.
The Journal of biological chemistry, 281(48), 37009-37016 (2006-10-07)
The biogenesis of spliceosomal small nuclear ribonucleoproteins (snRNPs) in higher eukaryotes requires the functions of several cellular proteins and includes nuclear as well as cytoplasmic phases. In the cytoplasm, a macromolecular complex containing the survival motor neuron (SMN) protein, Gemin2-8
Livio Pellizzoni et al.
Science (New York, N.Y.), 298(5599), 1775-1779 (2002-12-03)
The Survival of Motor Neurons (SMN) protein, the product of the spinal muscular atrophy-determining gene, is part of a large macromolecular complex (SMN complex) that functions in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). Using cell extracts and purified
Ashwin Chari et al.
Cell, 135(3), 497-509 (2008-11-06)
Spliceosomal small nuclear ribonucleoproteins (snRNPs) are essential components of the nuclear pre-mRNA processing machinery. A hallmark of these particles is a ring-shaped core domain generated by the binding of Sm proteins onto snRNA. PRMT5 and SMN complexes mediate the formation
Jennifer Baccon et al.
The Journal of biological chemistry, 277(35), 31957-31962 (2002-06-18)
The survival of motor neurons (SMN) protein is the product of the gene mutated or deleted in the neurodegenerative disease, spinal muscular atrophy. SMN is part of a large macromolecular complex that also contains Gemin2, Gemin3, Gemin4, Gemin5, and Gemin6.

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