G5165
Anti-Gαx/z antibody produced in rabbit
whole antiserum
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
contains
0.1% sodium azide
species reactivity
mammals
technique(s)
western blot: 1:100-1:200 using plasma membrane fractions
shipped in
dry ice
Storage temp.
−20°C
Gene Information
human ... GNAZ(2781)
mouse ... Gnaz(14687)
rat ... Gnaz(25740)
General description
G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and γ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands. The αz subunit is myristoylated near the N-terminus.
Immunogen
synthetic peptide corresponding to the C-terminal region of Gαx/z.
Application
Anti-Gαx/z antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:100-1:200 using plasma membrane fractions.
Biochem/physiol Actions
The gene GNAZ (guanine nucleotide binding protein (G protein), αz polypeptide) encodes an α subunit of guanine nucleotide binding proteins. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βγ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βγ-subunit complex. When coupled to phospholipase C, the α subunits may have a role in pertussis toxin-insensitive pathways.
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Storage Class
10 - Combustible liquids
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M Parenti et al.
The Biochemical journal, 291 ( Pt 2), 349-353 (1993-04-15)
We have examined the post-translational processing of G alpha subunits expressed endogenously in rat PC12 and NG108-15 rat/mouse hybrid cells, and after transfection of cDNA expression constructs into COS cells. Thioester-linked palmitoylation of alpha o, alpha s, alpha q/alpha 11
Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.
E H Hurowitz et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 7(2), 111-120 (2000-05-20)
Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Each subunit of the G protein complex is encoded by a member of one of three corresponding gene families. Currently, 16 different members
The many faces of G protein signaling.
H E Hamm
The Journal of biological chemistry, 273(2), 669-672 (1998-02-14)
M E Linder et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(8), 3675-3679 (1993-04-15)
A small number of membrane-associated proteins are reversibly and covalently modified with palmitic acid. Palmitoylation of G-protein alpha and beta subunits was assessed by metabolic labeling of subunits expressed in simian COS cells or insect Sf9 cells. The fatty acid
Theresa M Cabrera-Vera et al.
Endocrine reviews, 24(6), 765-781 (2003-12-13)
In multicellular organisms from Caenorhabditis elegans to Homo sapiens, the maintenance of homeostasis is dependent on the continual flow and processing of information through a complex network of cells. Moreover, in order for the organism to respond to an ever-changing
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