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G4252

Sigma-Aldrich

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym(s):

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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200 UNITS
$150.00
500 UNITS
$298.00
1000 UNITS
$596.00
2500 UNITS
$1,100.00

$150.00

Available to ship onApril 29, 2025Details

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200 UNITS
$150.00
500 UNITS
$298.00
1000 UNITS
$596.00
2500 UNITS
$1,100.00

About This Item

CAS Number:
9033-12-9
Enzyme Commission number:
4.4.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00131225
UNSPSC Code:
12352204
NACRES:
NA.54

$150.00

Available to ship onApril 29, 2025Details

type

Grade IV

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

≥400 units/mg protein

foreign activity

glyoxalase II ≤1%

storage temp.

2-8°C

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General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos.[1] It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.[2]

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.[3]

Unit Definition

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Analysis Note

Protein determined by biuret.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000401509
0000401509
0000290703
081M7670V
081M7670

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The mechanism of action of glyoxalase.
E RACKER
The Journal of biological chemistry, 190(2), 685-696 (1951-06-01)
An enzyme concerned with the formation of hydroxy acids from ketonic aldehydes
H.D. Dakin & H.W. Dudley
The Journal of Biological Chemistry, 14, 155-157 (1913)
Ravi Gupta et al.
Journal of proteome research, 11(5), 2684-2696 (2012-04-11)
Plants' distribution and productivity are adversely affected by low temperature (LT) stress. LT induced proteins were analyzed by 2-DE-nano-LC-MS/MS in shoot secretome of Hippophae rhamnoides (seabuckthorn), a Himalayan wonder shrub. Seedlings were subjected to direct freezing stress (-5 °C), cold
Miriam Urscher et al.
The FEBS journal, 279(14), 2568-2578 (2012-05-23)
Glucose consumption and therefore methylglyoxal production of human erythrocytes increase significantly upon infection with malaria parasites. The glyoxalase systems of the host-parasite unit cope with this metabolic challenge by catalyzing the removal of harmful methylglyoxal. Thus, glyoxalase 1 from the
Megan Jack et al.
Translational research : the journal of laboratory and clinical medicine, 159(5), 355-365 (2012-04-17)
Diabetic neuropathy is the most common and debilitating complication of diabetes mellitus with more than half of all patients developing altered sensation as a result of damage to peripheral sensory neurons. Hyperglycemia results in altered nerve conduction velocities, loss of
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