G3512

Glycerol Dehydrogenase from Cellulomonas sp.

lyophilized powder, ≥50 units/mg protein

• Synonym(s): Glycerol:NAD⁺ 2-oxidoreductase
• Enzyme Commission number: 1.1.1.6 (BRENDA, IUBMB)
• EC Number: 232-824-3
• MDL number: MFCD00131206
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Cellulomonas spp.)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: ~390 kDa
• composition: Protein, ≥60% biuret
• storage temp.: −20°C

Description

General description

Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family.[1] It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid.[2]

Application

Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method.[3] It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.[4][5]

This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD⁺ was catalyzed by the enzyme glycerol dehydrogenase.[6]

Biochem/physiol Actions

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone[7][8].

Physical properties

Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯²M (Glycerol), 8.9 x 10¯⁵M (NAD⁺⁾
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni⁺⁺, Cu⁺⁺, Zn⁺⁺, Cd⁺⁺)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K⁺, NH⁺4 and Rb⁺.

Unit Definition

One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.

Physical form

Lyophilized powder containing bovine serum albumin

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)