Biochemical and biophysical research communications, 492(3), 487-492 (2017-08-24)
The study combines HPLC-based with MS-based competitive analyses to evaluate the quenching activity of a set of carnosine derivatives towards methylglyoxal (MGO) and malondialdehyde (MDA) chosen as representative of α- and β-dicarbonyls, respectively. The obtained results underline that these derivatives
Amongst the available methodologies for protein determination, the bicinchoninic acid (BCA) assay highlights for its simplicity, sensitivity, repeatability and reproducibility. Nevertheless, in spite that the general principle behind this methodology is known, there are still unanswered questions regarding the chemistry
A glycine-histidine tag (Gly3His6) was added to the C-terminus of a fusion protein consisting of the cholera toxin B-subunit (CtxB) and the IgA protease beta-domain (Iga beta). The aim was to facilitate single-step purification and to create a suitable tool
Journal of inorganic biochemistry, 12(2), 143-156 (1980-04-01)
1H and 13C nmr studies on the Pd(II)Gly-His complex interaction with cytidine and GMP have shown that the nucleoside binds the palladium complex via N3 nitrogen and the nucleotide binds that complex via N7 nitrogen. The analysis of the Cyd
Knowledge of the complexes formed by N-coordinating ligands and Cu(II) ions is of relevance in understanding the interactions of this ion with biomolecules. Within this framework, we investigated Cu(II) complexation with mono- and polydentate ligands, such as ammonia, ethylenediamine (en)
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