F9762

Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD00162462
• UNSPSC Code: 12352203

Properties

• biological source: goat
• Quality Level: 200
• conjugate: FITC conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunofluorescence: suitable (1:32 - 1:64 using Hep2 cells)
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Human IgM (μ-chain specific)-FITC antibody specifically binds to mu-chain of human IgM. After immunospecific purification, anti-human IgM is conjugated to fluorescein isothiocyanate (FITC), in an alkaline reaction. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins. The product is also determined to have no cross reactivity with mouse or rat serum proteins.

Specificity

No cross-reaction with mouse and rat IgG.

Immunogen

Purified human IgM

Application

Anti-Human IgM (μ-chain specific)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:64 using human peripheral blood lymphocytes. A dilution of 1:32-1:64 may be used for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. A dilution of 1:32 may be used on acetone-fixed mouse liver cells for anti-nuclear antibody assay. It may also be used for agar diffusion assay.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)