F9429

Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-FBI-1, Anti-LRF, Anti-POK Erythroid Myeloid Ontogenic Factor, Anti-ZBTB7, Anti-Zinc Finger- and BTB Domain-containing Protein 7
• MDL number: MFCD08705458
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 75 kDa (additional band at 70 kDa may be seen)
• species reactivity: human
• technique(s): immunoprecipitation (IP): 1-2 μg using A431 cell lysates; microarray: suitable; western blot: 0.5-1 μg/mL using K-562 cell lysates
• UniProt accession no.: O95365
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ZBTB7A(51341)

Description

General description

FBI-1/Pokemon is a BTB/POZ containing zinc-finger protein. It is predominantly expressed in a subset of T-cell lymphoma and B-cell lymphomas. It is also found in breast, lung, colon, prostate, and bladder carcinomas. It was identified as a critical factor in oncogenesis and is called Pokemon (POK erythroid myeloid ontogenic factor).

Immunogen

synthetic peptide corresponding to amino acids 570-584 of human FBI-1/Pokemon, conjugated to KLH.

Application

Anti-FBI-1/Pokemon (C-terminal) antibody is suitable for immunoprecipitation at concentration of 1-2μg using A431 cell lysates, microarray and western blot at concentration of 0.5-1μg/mL using K-562 cell lysates.

Biochem/physiol Actions

FBI-1/Pokemon (C-Terminal) act as potent transcriptional repressors through the recruitment of histone deacetylases (HDACs) and subsequent chromatin remodeling. By binding to the tumor suppressor gene ARF, the Pokemon exerts its oncogenic activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)