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F8649

Sigma-Aldrich

Formate Dehydrogenase from Candida boidinii

lyophilized powder, 5.0-15.0 units/mg protein

Synonym(s):

FDH, Formate:NAD+ oxidoreductase

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$281.00
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$984.00
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$1,670.00

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50 UNITS
$281.00
250 UNITS
$984.00
500 UNITS
$1,670.00

About This Item

CAS Number:
9028-85-7
Enzyme Commission number:
1.2.1.2 (BRENDA, IUBMB)
EC Number:
232-844-2
MDL number:
MFCD00081614
UNSPSC Code:
12352204
NACRES:
NA.54

$281.00

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Request a Bulk Order

biological source

fungus (Candida boidinii)

Quality Level

200

form

lyophilized powder

specific activity

5.0-15.0 units/mg protein

composition

Protein, 5.0-20.0% biuret

storage temp.

2-8°C

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Application

Formate Dehydrogenase (FDH) is used for diagnostics in large scale industrial processes. Its used in the production of an unnatural amino acid, tert-L-leucine, a component of some HIV protease and matrix metalloprotease inhibitors.[1]

Biochem/physiol Actions

Formate dehydrogenase is an abundant enzyme from yeast Candida boidinii (CbFDH) that plays an important role in the energy supply of methylotrophic microorganisms and in the stress response of plants.[1]
Formate dehydrogenase is involved in the stress response of plants [2] and catalyzes the reduction of NAD+ to NADH.[3]

Unit Definition

One unit will oxidize 1.0 μmole of formate to CO2 per min in the presence of β-NAD at pH 7.6 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG4161
SLCF4323
SLCF0381
SLCD5061
SLCD2542

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Do reactive oxygen species or does oxygen itself confer obligate anaerobiosis? The case of Bacteroides thetaiotaomicron.
Khademian, et al.
Molecular Microbiology, 114, 333-347 (2021)
Held in police custody.
A Skelt
Nursing times, 84(4), 50-52 (1988-01-02)
Jana Löwe et al.
Scientific reports, 8(1), 10436-10436 (2018-07-12)
A biotechnological process is reported, which enables an enzymatic reduction without the need for addition of an organic co-substrate for in situ-cofactor recycling. The process is based on merging the fields of enzymatic reductive amination with formate dehydrogenase-based in situ-cofactor
Frances L Shaw et al.
Journal of bacteriology, 194(15), 3814-3823 (2012-05-23)
The food-borne bacterial pathogen Campylobacter jejuni efficiently utilizes organic acids such as lactate and formate for energy production. Formate is rapidly metabolized via the activity of the multisubunit formate dehydrogenase (FDH) enzyme, of which the FdhA subunit is predicted to
V I Tishkov et al.
Biochemistry. Biokhimiia, 69(11), 1252-1267 (2005-01-04)
NAD+-dependent formate dehydrogenase (FDH) is an abundant enzyme that plays an important role in energy supply of methylotrophic microorganisms and in response to stress in plants. FDH belongs to the superfamily of D-specific 2-hydroxy acid dehydrogenases. FDH is widely accepted
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Questions

1–4 of 4 Questions  

  1. Could you please provide the uniprot accession code of the enzyme? Best regards

    1 answer

    1. The UniProt accession ID is O13437. Please access this link below:
      https://www.uniprot.org/uniprotkb/O13437/entry.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What solvent can Product F8649, Formate dehydrogenase from Candida boidinii be  dissolved in?

    1 answer

    1. Product F8649, Formate dehydrogenase from Candida boidinii can be dissolved in 50 mM phosphate buffer, pH 7.5 to 8.0, or in double distilled water. Solubility is approximately 10 mg/ml.

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  4. How do you store Formate Dehydrogenase from Candida boidinii , product F8649, once it has been solublized?

    1 answer

    1. Aliquots of the enzyme Formate Dehydrogenase from Candida boidinii , product F8649, should be stable for at least 6 months when stored at -15 to -25°C, at +33°C for 1 day, and at +2 to +8°C for 7 days. Aliquots should be thawed only once.

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