Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells
Storage and Stability
To effectively preserve the integrity of animal serum, it should be stored frozen and protected from light. Multiple thaw/freeze cycles should be avoided as they will hasten the degradation of serum nutrients and can result in the formation of insoluble precipitates. For stability and optimal performance, serum should be stored at −20 °C and used prior to the labeled expiration date.
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