F8051
Anti-phospho-FAK (pTyr407) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-phospho-Focal Adhesion Kinase
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 125 kDa
species reactivity
chicken, mouse, rat, frog, human
technique(s)
western blot: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... PTK2(5747)
mouse ... Ptk2(14083)
rat ... Ptk2(25614)
General description
Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase that is expressed ubiquitously. FAK is the critical in forming cell-substratum adhesions or focal adhesion complexes. FAK reportedly has multiple phosphorylation sites. Autophosphorylation of FAK at Tyr397 creates a binding site on FAK for Src-family kinases and is critical for maximum adhesion and migration responses. The phosphorylation of Tyr407 and Tyr861 is induced during epithelial-mesenchymal transition and further augmented during cell migration. FAK activation couples signal transduction via PI3K, CAS and Src. FAK signaling is involved in multiple functions such as proliferation, migration, survival and angiogenesis. Disruption of FAK signalling results in failure of adhesion to the substratum and anoikis (apoptosis) of epithelial cells which are anchorage dependent. FAK has been observed to be upregulated in human tumors such as neuroblastoma and is related to tumor cell survival and prognosis
Anti-phospho-FAK (pTyr407) recognizes FAK (Focal Adhesion Kinase) phosphorylated at tyrosine 407 (125 kDa).
Anti-phospho-FAK (pTyr407) recognizes FAK (Focal Adhesion Kinase) phosphorylated at tyrosine 407 (125 kDa).
Immunogen
synthetic phosphopeptide derived from the region of FAK that contains tyrosine 407.
Application
A starting dilution of 1:1000 is suitable for detection of FAK phosphorylated at tyrosine 407 by immunoblotting.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without magnesium and calcium), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL bovine serum albumin (IgG, protease free) as a carrier and 0.05% sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Protein kinase B/AKT and focal adhesion kinase: two close signaling partners in cancer
Wang S and Basson MD
Anticancer Agents in Medicinal Chemistry, 10, 993-1002 (2011)
Lauren Gillory et al.
Anti-cancer agents in medicinal chemistry, 10(10), 714-721 (2011-01-29)
Neuroblastoma is the most common extracranial solid tumor encountered in children, and continues to carry a dismal prognosis. Focal adhesion kinase (FAK) has been shown to be upregulated in a number of human tumors and is related to tumor virulence
Tanguy Lechertier et al.
The Journal of pathology, 226(2), 404-412 (2011-10-11)
Angiogenesis, the formation of new blood vessels from pre-existing ones, is essential for tumour development. It is initiated and regulated by growth factors via their surface receptors, which activate several intracellular signalling pathways in endothelial cells. Cell adhesion molecules, such
Jihe Zhao et al.
Cancer metastasis reviews, 28(1-2), 35-49 (2009-01-27)
Cellular interactions with extracellular matrix play essential roles in tumor initiation, progression and metastasis. Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase identified as a key mediator of signaling by integrins, a major family of cell surface receptors for
Marta Canel et al.
Cancer research, 70(22), 9413-9422 (2010-11-04)
Most cancer-related deaths are due to the development of metastatic disease, and several new molecularly targeted agents in clinical development have the potential to prevent disease progression. However, it remains difficult to assess the efficacy of antimetastatic agents in the
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