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F7805

Sigma-Aldrich

FER (542-end), active, GST tagged from mouse

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

AV062135, C330004K01 Rik, Fert, Fert2

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About This Item

UNSPSC Code:
12352200

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

557-753 nmol/min·mg

mol wt

~59 kDa

UniProt accession no.

P70451

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Fert2(14158)

General description

FER is a nonreceptor protein-tyrosine kinase (PTK), which has a ubiquitous expression pattern. Along with Fps/Fes protein, it makes up the subfamily called PTK. It contains three predicted coiled-coil (CC) motifs in its N-terminal, a Src homology 2 (SH2) domain located in the center, and a catalytic domain at its C-terminal. An isoform of this protein is specific to the testis, and is called FerT.[1]

Biochem/physiol Actions

In fibroblasts, FER acts downstream of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) receptors. It is also activated downstream of FcγRI, a high-affinity receptor for immunoglobulin E (IgE), in mast cells. However, the exact role in these pathways is yet unknown. Inactivation of this protein in mice results in reduction in cortactin phosphorylation, though they are viable and fertile. Thus, Fer might be involved in controlling cell growth, adhesion, development of retina and spermatogenesis. It is essential for the phosphorylation of cortactin.[1]

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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O Pasder et al.
Oncogene, 25(30), 4194-4206 (2006-05-30)
Fer is a nuclear and cytoplasmic intracellular tyrosine kinase. Herein we show that Fer is required for cell-cycle progression in malignant cells. Decreasing the level of Fer using the RNA interference (RNAi) approach impeded the proliferation of prostate and breast
Peter Greer
Nature reviews. Molecular cell biology, 3(4), 278-289 (2002-05-08)
Fps/Fes and Fer are the only known members of a distinct subfamily of the non-receptor protein-tyrosine kinase family. Recent studies indicate that these kinases have roles in regulating cytoskeletal rearrangements and inside out signalling that accompany receptor ligand, cell matrix
A W Craig et al.
Molecular and cellular biology, 21(2), 603-613 (2001-01-03)
The ubiquitous Fer protein-tyrosine kinase has been proposed to regulate diverse processes such as cell growth, cell adhesion, and neurite outgrowth. To gain insight into the biological function of Fer, we have targeted the fer locus with a kinase-inactivating missense

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