F7305
Fetal Bovine Serum
Mouse Embryonic Stem Cell Screened, suitable for cell culture, sterile-filtered, USDA Approved
Synonym(s):
FBS, FCS, sera, serum
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About This Item
MDL number:
UNSPSC Code:
12352207
biological source:
bovine fetus
sterility:
sterile-filtered
shipped in:
dry ice
Recommended Products
biological source
bovine fetus
sterility
sterile-filtered
quality
USDA Approved
composition
Hemoglobin, ≤25 mg/dL
technique(s)
cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
Storage temp.
−20°C
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General description
Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting balanced growth-promoting and growth-inhibiting functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.[1][2]
Most Fetal Bovine Serum (FBS) batches do not support mouse ES cells. Sigma-Aldrich has pre-screened multiple FBS batches to increase your chances of selecting a batch that will perform within your laboratory, saving you time and money.
In addition to our extensive Fetal Bovine Serum performance and quality assays (mycoplasma, sterility, endotoxin, hemoglobin, etc.), we have developed low density, feeder-free assays to assess Fetal Bovine Serum′s support of mouse embryonic stem cells. Only those batches that performed well with mouse ES cells were selected for this product.
Most Fetal Bovine Serum (FBS) batches do not support mouse ES cells. Sigma-Aldrich has pre-screened multiple FBS batches to increase your chances of selecting a batch that will perform within your laboratory, saving you time and money.
In addition to our extensive Fetal Bovine Serum performance and quality assays (mycoplasma, sterility, endotoxin, hemoglobin, etc.), we have developed low density, feeder-free assays to assess Fetal Bovine Serum′s support of mouse embryonic stem cells. Only those batches that performed well with mouse ES cells were selected for this product.
Application
FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Analysis Note
- Endotoxin and hemoglobin tested
- Mouse embryonic stem cell screened
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Robert C Tuckey et al.
The Journal of steroid biochemistry and molecular biology, 112(4-5), 213-219 (2008-11-13)
Cytochrome P450scc (CYP11A1) metabolizes vitamin D3 to 20-hydroxyvitamin D3 as the major product, with subsequent production of dihydroxy and trihydroxy derivatives. The aim of this study was to determine whether cytochrome P450scc could metabolize 1alpha-hydroxyvitamin D3 and whether products were
Perrine Barraud et al.
BMC neuroscience, 9, 24-24 (2008-02-20)
The olfactory epithelium is a neurogenic tissue comprising a population of olfactory receptor neurons that are renewed throughout adulthood by a population of stem and progenitor cells. Because of their relative accessibility compared to intra-cranially located neural stem/progenitor cells, olfactory
Thi A Tran et al.
Journal of neuroinflammation, 5, 14-14 (2008-05-14)
Recent animal and human studies implicate chronic activation of microglia in the progressive loss of CNS neurons. The inflammatory mechanisms that have neurotoxic effects and contribute to neurodegeneration need to be elucidated and specifically targeted without interfering with the neuroprotective
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