The DAPI concentration is 1.5 ug/mL.
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Product Name
Fluoroshield™ with DAPI, liquid
description
histology mounting medium
Quality Level
form
liquid
composition
Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl
technique(s)
immunofluorescence: suitable
pH range
7.9-8.3
solubility
water: soluble
fluorescence
λex 360 nm; λem 460 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
General description
Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.
Application
Legal Information
FluoroShield is a trademark of ImmunoBioScience Corp.
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the concentration of DAPI in the Fluoroshield?
1 answer-
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What could be the reason for the occurrence of a white film after curing, particularly when the coverslips are slightly wet with PBS?
1 answer-
The following recommendations are provided:
•If there is tissue attached to glass slides, it is recommended to use a Kimwipe to carefully dry the slide around the tissue section.
•If cells are on a coverslip, removing excess water from the coverslip by placing it on its end and then using a paper towel to wick away excess moisture is suggested.
•The possibility that as the slides dry, salts from the PBS are evaporating and leaving a white residue or film is considered.Helpful?
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How is Fluoroshield (F6057) used with DAPI?
1 answer-
Fluoroshield with DAPI is an aqueous mounting medium designed to preserve the fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of various fluorescent dyes. Additionally, it retains fluorescence during prolonged storage at 4°C in the dark. Importantly, the medium does not contain phenylenediamine, which can destroy immunofluorescence of certain dyes. The mounting medium is fortified with DAPI, a counter-stain for DNA, intended for use in situ hybridization techniques or other methods requiring fluorescence of DNA staining. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. The intended use of this product includes immunofluorescence and confocal microscopy.
Reagent:
Fluoroshield with DAPI is a ready-to-use mounting medium.Storage:
It should be stored at 2-8°C, protected from light, and should not be frozen.Procedure:
- Bring the vial to room temperature.
- Rinse the slide to be mounted with distilled or deionized water, remove excess water by touching the edges of the slide on a paper towel, and place the slides on a flat surface away from light.
- Apply 3-4 drops of mounting medium directly on top of the specimen and spread it out evenly, ensuring the tissue is not touched. Excess medium can be removed by touching the edges of the slide on a paper towel.
- Let it stand at room temperature for about 5 minutes.
- Apply a cover slip, carefully avoiding air bubbles. If a coverslip is not used, air bubbles will appear in a few days.
- For long-term storage, it is recommended to store the slide in the dark at 2-8°C.Removal of Coverslip:
The coverslip can be removed before sealing the edges. Soak the slide in warm (37°C) water for a few minutes, carefully and slowly moving.Helpful?
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Is sealing a 96-well plate necessary in the final step of ICC with Fluoroshield™ with DAPI, as done with coverslips using clear nail polish?
1 answer-
Fluoroshield with DAPI is designed for use with glass slides or coverslips intended for analysis using a fluorescent microscope. It is not commonly used for coverslipping 96 well plates. There are no known individual, small, round coverslips for each of the 96 wells, nor are there large clear glass plates to fit over the top of a 96 well plate. If such glass plates are available, it is not known that such a product exists.
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What are the potential implications of storing a bottle of F6057-20 at -20°C for 2 days by mistake?
1 answer-
It is suggested to allow the bottle to thaw. Once thawed, the bottle should be inverted several times to thoroughly mix its contents. If the solution looks normal and does not contain any precipitate or particulate matter, it is expected that the mounting media is suitable for use.
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What are the procedures for solubilizing and using Fluoroshield with DAPI (F6057)?
1 answer-
Fluoroshield with DAPI is a solution that is ready to use and does not require solubilization. It is an aqueous mounting medium designed to preserve the fluorescence of tissue and cell smears. The mounting medium is fortified with DAPI, a counter-stain for DNA, and is intended for use in situ hybridization techniques, immunofluorescence and confocal microscopy.
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what is the refractive index？
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The refractive index is not determined internally for this product. However, the supplier reports that it is 1.364 ± 0.002 of the product in solution.
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Is Fluoroshield with DAPI (F6047) compatible for high-maginification microscopy or is it recommended to use hard-setting medium?
1 answer-
This product has been used for cell imaging. Please see the following protocols and review the pee-reviewed articles:
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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