F4129

Fibrinogen from human plasma

55-75% protein, ≥60% of protein is clottable

• Synonym(s): Factor I
• CAS Number: 9001-32-5
• EC Number: 232-598-6
• MDL number: MFCD00131060
• UNSPSC Code: 12352202

Properties

• biological source: human plasma
• form: powder
• quality: ≥60% of protein is clottable
• mol wt: α-chain 63.5 kDa; β-chain 56 kDa; γ chain 47 kDa (about 4% carbohydrate content); soluble dimer 340 kDa
• concentration: 55-75% protein (biuret); 55-75% protein
• solubility: 0.9% NaCl: soluble 10 mg/mL
• UniProt accession no.: P02671; P02675; P02679
• Storage temp.: −20°C
• Gene Information: human ... FGA(2243) , FGB(2244) , FGG(2266)

Description

General description

Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa.

Application

Fibrinogen was used in velocity variation assessment of red blood cell aggregation using spectral domain Doppler optical coherence tomography.[1] It was also used to study the nano-bio interaction between graphite oxide nanoparticles and human blood components.[2]

Biochem/physiol Actions

Acute phase protein that is part of the coagulation cascade of proteins.

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Specifications

Source material has tested negative for HIV and HBsAg.

Physical form

Lyophilized powder containing ~15% sodium citrate and ~20% sodium chloride.

Analysis Note

Protein determined by biuret

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)