F4125
Ficin from fig tree latex
saline suspension, ≥1.0 units/mg protein (biuret)
Synonym(s):
Debricin, Ficain, higueroxyl delabarre
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100 UNITS
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200 UNITS
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100 UNITS
$176.00
200 UNITS
$282.00
1000 UNITS
$3,500.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$176.00
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Recommended Products
biological source
fig tree (latex)
Quality Level
form
saline suspension
specific activity
≥1.0 units/mg protein (biuret)
mol wt
23.8 kDa
storage temp.
2-8°C
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General description
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0
pI: 9.0
Application
Biochem/physiol Actions
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).
Physical form
Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0
Preparation Note
2× Crystallized
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Ficin (EC 3.4.22.3), a cysteine proteinase isolated from the latex of a Ficus tree, is known to occur in multiple forms. Although crude ficin is of considerable commercial importance, ficin as such has not been fully characterized. A major ficin
Protocols
This procedure may be used for all Ficin products.
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