F3520

Anti-von Willebrand Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Von Willebrand Factor Antibody, Von Willebrand Factor Antibody - Anti-von Willebrand Factor antibody produced in rabbit, Anti-Factor VIII-Related Antigen
• MDL number: MFCD00162556
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 300
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling); indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue
• UniProt accession no.: P04275
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VWF(7450)

Description

General description

Anti-von Willebrand Factor is produced in rabbit using purified von Willebrand factor (factor VIII R:Ag) as the immunogen.

Human von Willebrand factor (factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Specificity

The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections.

Immunogen

von Willebrand Factor (Factor VIII-related antigen) from human plasma.

Application

Anti-von Willebrand Factor antibody produced in rabbit has been used for:

• western blot analysis and immunostaining
• immunocytochemistry
• immunohistochemistry
• indirect immunofluorescence

Biochem/physiol Actions

It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Whole antiserum is purified to provide an IgG fraction of antiserum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable