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Key Documents

F2561

Sigma-Aldrich

Fetal Bovine Serum

sterile-filtered, suitable for cell culture

Synonym(s):

FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352200

biological source

bovine fetus

sterility

sterile-filtered

product line

FBS Classic

composition

Hemoglobin, ≤25 mg/dL

origin

Brazil origin

availability

available only in Brazil

technique(s)

cell culture | mammalian: suitable

impurities

≤5 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting balanced growth-promoting and growth-inhibiting functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Fetal bovine serum (FBS) is suitable for broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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FT-IR characterization of a theranostic nanoprobe for photodynamic therapy and epidermal growth factor receptor targets.
Castilho ML et al.
Sensors and Actuators B, Chemical, 240, 903-908 (2017)
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Barbara Belletti et al.
Molecular biology of the cell, 19(5), 2003-2013 (2008-02-29)
The balanced activity of microtubule-stabilizing and -destabilizing proteins determines the extent of microtubule dynamics, which is implicated in many cellular processes, including adhesion, migration, and morphology. Among the destabilizing proteins, stathmin is overexpressed in different human malignancies and has been
Silvia Goldoni et al.
The American journal of pathology, 173(3), 844-855 (2008-08-09)
Decorin, a member of the small leucine-rich proteoglycan gene family, down-regulates members of the ErbB receptor tyrosine kinase family and attenuates their signaling, leading to growth inhibition. We investigated the effects of decorin on the growth of ErbB2-overexpressing mammary carcinoma
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