F1555
Anti-FOXA1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Forkhead box A1, Anti-HNF3A, Anti-Hepatocyte nuclear factor 3α, Anti-MGC33105, Anti-TCF3A
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
human
technique(s)
indirect immunofluorescence: 1:100-1:200 using MCF7 cells fixed with paraformaldehyde-Triton
western blot: 1:250-1:500 using extracts of the MCF7 cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FOXA1(3169)
General description
FOXA1 gene is mapped to human chromosome 14q21.1. FOXA1 belongs to the FOXA family. Forkhead proteins comprise “winged helix” DNA binding or forkhead domain.[1]
Forkhead box protein A1 (FOXA1) is a protein encoded by the FOXA1 gene in humans and is a member of the FOX family of transcription factors.
Specificity
Anti-FOXA1 specifically recognizes human FOXA1.
Immunogen
synthetic peptide corresponding to amino acids 441-459 of human FOXA1, conjugated to KLH via a N-terminal cysteine residue.
Application
Anti-FOXA1 antibody produced in rabbit has been used in immunoblotting.[1]
Anti-FOXA1 antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:100-1:200 using MCF7 cells fixed with paraformaldehyde-Triton. It is also suitable for western blotting at a dilution of 1:250-1:500 using extracts of the MCF7 cell line.
Biochem/physiol Actions
FOXA group of proteins are referred to as “pioneer factors” as they interact with compacted chromatin and make them accessible for transcription factors.[1] FOXA1 is a crucial partner in events that lead to breast cancer progression in connection with estrogen receptor (ER).
Forkhead box protein A1 (FOXA1) exhibits the greatest extent of mitotic chromosome binding. FOXA1 is involved in embryonic development and cell differentiation. It promotes cell proliferation by androgen receptor (AR) and activates Notch pathway.[2] FOXA1 acts as a negative regulator in the initial stages of lung cancer metastasis. It may modulate the epithelial-to-mesenchymal transition through its transcriptional regulatory network. FOXA1 has been implicated in certain tumor types including breast, prostate, lung, thyroid and esophageal squamous cell carcinomas. It might act as a potential regulator of progression of human glioma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Haiyun Wang et al.
BMC genomics, 14, 680-680 (2013-10-08)
The epithelial-to-mesenchymal transition is an important mechanism in cancer metastasis. Although transcription factors including SNAIL, SLUG, and TWIST1 regulate the epithelial-to-mesenchymal transition, other unknown transcription factors could also be involved. Identification of the full complement of transcription factors is essential
Lan Song et al.
Cell stress & chaperones, 19(1), 53-60 (2013-04-30)
Forkhead box protein A1 (FoxA1) is a transcription factor that is involved in embryonic development and cell differentiation. In this study, we show that hydrogen peroxide (H2O2) treatment upregulated expression of FoxA1 and UCP2 in the A549 cell line. Overexpression
Jessica L L Robinson et al.
Frontiers in oncology, 3, 20-20 (2013-02-20)
The forkhead protein, FOXA1, is a critical interacting partner of the nuclear hormone receptors, oestrogen receptor-α (ER) and androgen receptor (AR), which are major drivers of the two most common cancers, namely breast and prostate cancer. Over the past few
Liang Wang et al.
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, 20(5), 654-658 (2013-03-21)
Forkhead-box A1 (FOXA1), a member of the FOX family of transcription factors, has been implicated in certain tumor types including breast, prostate, lung, thyroid and esophageal squamous cell carcinomas. The aim of this study was to investigate the clinicopathological significance
Meiting Qiu et al.
BMC cancer, 14, 78-78 (2014-02-12)
Increasing evidence suggests that forkhead box A1 (FOXA1) is frequently dysregulated in many types of human cancers. However, the exact function and mechanism of FOXA1 in human endometrial cancer (EC) remains unclear. FOXA1 expression, androgen receptor (AR) expression, and the
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