F0675

Anti-Fractalkine antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-CX3CL1
• MDL number: MFCD01864668
• UNSPSC Code: 51111800
• NACRES: NA.41

Properties

• biological source: goat
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL using human tissue; western blot: 0.1-0.2 μg/mL
• UniProt accession no.: P78423
• storage temp.: −20°C
• Gene Information: human ... CX3CL1(6376)

Description

General description

Fractalkine, also known as neurotactin is an atypical chemokine, synthesized as a membrane-bound molecule containing a chemokine domain presented on a mucin-like stalk which facilitates the direct capture of circulating leukocytes. It also has a crucial role in all stages of atherogenesis like recruitment, retention and survival of monocytes in early atherosclerotic lesions along with the foam cell persistence in advanced plaques. Anti-Fractalkine antibody can be used in neutralization of biological activity of recombinant human fractalkine. It can also be used in immunohistochemistry. Goat anti-Fractalkine antibody has shown 35% (approx.) cross-reactivity with recombinant mouse fractalkine/CX3CL1 and 15% (approx.) cross-reactivity with recombinant rat CX3CL1. This antibody also has 5% cross-reacitivity with recombinant human eotaxin, recombinant human MCP-1, recombinant mouse MARC, recombinant human MCP-2, recombinant human 6Ckine and recombinant mouse eotaxin.

Specificity

The antibody will neutralize the biological activity of recombinant human fractalkine/CX3CL1. Based on ELISA, the antibody shows ~35% cross-reactivity with recombinant mouse fractalkine/CX3CL1 and 15% cross-reactivity with recombinant rat CX3CL1. The antibody also shows 5% cross-reacitivity with recombinant human eotaxin, recombinant mouse eotaxin, recombinant human MCP-1, recombinant human MCP-2, recombinant human 6Ckine, and recombinant mouse MARC.

Immunogen

recombinant human fractalkine/CX3CL1 chemokine domain, expressed in E. coli.

Application

Anti-Fractalkine antibody can be used in western blotting and ELISA.

Physical form

Lyophilized from 0.2 μm filtered solution of phosphate buffered saline with 5% trehalose.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable