E9413

Cytochrome P450 2D6 human

• Synonym(s): Cytochrome P450 Microsome Preparation human, Cerosomes
• Enzyme Commission number: 1.14.14.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in Saccharomyces cerevisiae
• form: liquid
• specific activity: ≥10 units/pmol enzyme
• secondary activity: ≥100 units/mg protein (Reductase activity)
• concentration: ≥10 mg/mL protein (Bradford); 1 pmol/μL
• UniProt accession no.: P10635
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP2D6(1565)

Description

General description

Microsome contents of CYP2D6 human and yeast CYP-reductase expressed in Saccharomyces cerevisiae.

Application

Recombinant cytochrome P450, isozyme 2D6, (CYP2D6) may be used in toxicity and drug metabolism/ pharmacokinetics research to identify its specificity and activity versus xenobiotic and endogenous compounds. Recombinant cytochrome P450, isozyme 2D6, may be used separately or as a component of a P450 isozyme panel to identify and characterize natural and synthetic selective inhibitors that affect the metabolism of toxic compounds or therapeutic drugs such as fluoxetine, codeine, and tamoxifen.

Unit Definition

P450 Activity: One unit will oxidize 1 picomole of dextromethorphan per minute at pH 7.4 at 28 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.

Physical form

50 mM Tris (pH 7.4), 1 mM EDTA, 20% glycerol

Other Notes

For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable