E9288

Cytochrome P450 2E1 human

recombinant, expressed in Saccharomyces cerevisiae

• Synonym(s): Cytochrome P450 Microsome Preparation human, Cerosomes
• EC Number: 1.14.14.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in Saccharomyces cerevisiae
• form: liquid
• specific activity: ≥5 units/pmol enzyme (P450 activity)
• secondary activity: ≥500 units/mg protein (reductase activity)
• concentration: ≥10 mg/mL protein (Bradford); 1 pmol/μL
• UniProt accession no.: P05181
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CYP2E1(1571)

Description

General description

Microsome contains CYP2E1 human and yeast CYP-reductase expressed in Saccharomyces cerevisiae.

Application

Recombinant cytochrome P450, isozyme 2E1, (CYP2E1) may be used in toxicity and drug metabolism/pharmacokinetics research to identify its specificity and activity versus xenobiotic and endogenous compounds. Recombinant cytochrome P450, isozyme 2E1, may be used separately or as a component of a P450 isozyme panel to identify and characterize natural and synthetic selective inhibitors that affect the metabolism of toxic compounds or therapeutic drugs.

Unit Definition

P450 Activity: One unit will oxidize 1 picomole of p-nitrophenol per minute at pH 7.4 at 28 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.

Physical form

50 mM Tris (pH 7,4), 1 mM EDTA, 20% glycerol

Other Notes

For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable