E8402
EphA5/Fc Chimera from rat
>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Synonym(s):
Bsk, Cek7, Ehk1, Hek7, Rek7
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
rat
recombinant
expressed in NSO cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
mol wt
monomer calculated mol wt 84 kDa
~110 kDa by SDS-PAGE (reducing)
packaging
pkg of 200 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
ligand binding assay: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Epha5(79208)
General description
EphA5 (ephrin receptor A5) is a receptor tyrosine kinase, which is a member of the ephrin receptor family. This family is divided into two classes A and B. EphA5 belongs to EphA group, which contains 10 members. The ligands to this group of receptors are glycosylphosphatidylinositol (GPI)-anchored named ephrin-A1-A6.[1] It is a highly regulated gene, and is expressed in both developing and adult central nervous system. In rat brain, three variants of EphA5 mRNA are found, with sizes 8.5, 5.9 and 5.1kb. In adult brain, it is expressed in deep cerebellar nuclei in the large neurons, hippocampus, cerebral cortex, and in cerebellum in the Purkinje and granular cells.[2]
Biochem/physiol Actions
EphA5 (ephrin receptor A5) is involved in the maintenance and development of differentiated neurons in the adult central nervous system.[2] In ventromedial hypothalamus, alterations in synaptic plasticity and interactions of EphA5 might result in aberration of glucagon secretion, and responses to recurrent hypoglycemia.[3] It is involved in cellular response to irradiation/ genotoxic stimuli, and lung cancer cells with inactivated EphA5 have aberrant G1/S cell cycle checkpoint. This protein is also up-regulated in lung cancer cells.[4]
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, and ephrin-A5; involved in pattern formation and morphogenesis.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Analysis Note
The biological activity is measured by its ability to bind recombinant mouse ephrin-A2/Fc in an ELISA assay.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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V Taylor et al.
Neuroscience, 63(1), 163-178 (1994-11-01)
Protein tyrosine kinases are pivotal in central nervous tissue development and maintenance. Here we focus on the expression of Ehk-1, a novel Elk-related receptor tyrosine kinase. Ehk-1 gene expression is observed in the developing and adult central nervous system and
Fernanda I Staquicini et al.
The Journal of biological chemistry, 290(12), 7345-7359 (2015-01-28)
Lung cancer is often refractory to radiotherapy, but molecular mechanisms of tumor resistance remain poorly defined. Here we show that the receptor tyrosine kinase EphA5 is specifically overexpressed in lung cancer and is involved in regulating cellular responses to genotoxic
Barbara Szepietowska et al.
Diabetes, 63(3), 1140-1147 (2013-11-14)
Hypoglycemia stimulates counterregulatory hormone release to restore euglycemia. This protective response is diminished by recurrent hypoglycemia, limiting the benefits of intensive insulin treatment in patients with diabetes. We previously reported that EphA5 receptor-ephrinA5 interactions within the ventromedial hypothalamus (VMH) influence
Xuelu Huan et al.
PloS one, 8(9), e74040-e74040 (2013-10-03)
The 16 EphA and EphB receptors represent the largest family of receptor tyrosine kinases, and their interactions with 9 ephrin-A and ephrin-B ligands initiate bidirectional signals controlling many physiological and pathological processes. Most interactions occur between receptor and ephrins of
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