One unit corresponds to the amount of enzyme which liberates 1 μmol of p-nitroaninline per minute at pH 7.0 at 25° C, using the substrate Z-Gly-Pro-pNA.
Application
Endoproteinase Pro-C has been used for protein analysis. It acts as a catalyst in the formation of Pro-X bonds in frozen aqueous systems.[1]
Biochem/physiol Actions
Endoproteinase Pro-C catalyzes Pro-Leu bond formation.[1]
Unit Definition
One unit corresponds to the amount of enzyme which liberates 1 μmol of p-nitroaninline per minute at pH 7.0 at 25° C, using the substrate Z-Gly-Pro-pNA.
Endoproteinase Pro-C catalyzed Peptide Bond Formation in Frozen Aqueous Systems.
Enz. Microbiol. Technol., 22(7), 617-620 (1998)
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