E7032
EPHB1 (591-end), active, GST tagged from mouse
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
9330129L11, AW488255, EPHT2, Elk, Elkh, Hek6, Net
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
122-165 nmol/min·mg
mol wt
~62 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... Ephb1(270190)
General description
EPHB1 (Ephb1 Eph receptor B1) is a glutamatergic synapse forming tyrosine kinase. Mammals contain five EPHB receptors, namely, EPHB1-EPHB4 and EPHB6. Their ligands are transmembrane in nature and are called ephrinB1, -B2 and -B3.
Application
EPHB1 (591-end), active, GST tagged from mouse has been used for in vitro phosphorylation of Caskin1.
Biochem/physiol Actions
EPHB1 (Ephb1 Eph receptor B1) is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis. This receptor is essential for the maintenance of hyperalgesia, and is the predominant receptor for chronic pain in the spinal cord.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Cecile Vindis et al.
The Journal of cell biology, 162(4), 661-671 (2003-08-20)
Eph receptors and their ligands (ephrins) play an important role in axonal guidance, topographic mapping, and angiogenesis. The signaling pathways mediating these activities are starting to emerge and are highly cell- and receptor-type specific. Here we demonstrate that activated EphB1
Cécile Vindis et al.
The Journal of biological chemistry, 279(27), 27965-27970 (2004-04-27)
Interactions between Eph receptors and their membrane-bound ligands (ephrins) are of critical importance for key developmental processes such as boundary formation or vascular development. Their downstream signaling pathways are intricate and heterogeneous at several levels, the combined effect being a
Szabolcs Pesti et al.
Cell communication and signaling : CCS, 10(1), 36-36 (2012-11-28)
Scaffold proteins have an important role in the regulation of signal propagation. These proteins do not possess any enzymatic activity but can contribute to the formation of multiprotein complexes. Although scaffold proteins are present in all cell types, the nervous
Vincent Cibert-Goton et al.
PloS one, 8(1), e53673-e53673 (2013-01-24)
EphB receptors tyrosine kinases and ephrinB ligands were first identified as guidance molecules involved in the establishment of topographical mapping and connectivity in the nervous system during development. Later in development and into adulthood their primary role would switch from
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