E7031
Anti-EZH2 (N-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-ENX-1, Anti-Enhancer of zeste homolog 2 (Drosophila)
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 100 kDa
species reactivity
mouse, human
technique(s)
western blot: 1:500-1:1,000 using K562 cell lysates
western blot: 1:500-1:1,000 using NIH3T3 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EZH2(2146)
mouse ... Ezh2(14056)
General description
EZH2 gene is mapped to human chromosome 7q36.1.
Histone-lysine N-methyltransferase EZH2 is an enzyme encoded by the EZH2 gene in humans. It belongs to the multiprotein polycomb repressive complex 2.
Specificity
Anti-EZH2 (N-terminal) recognizes human and mouse EZH2.
Immunogen
synthetic peptide corresponding to amino acids 1-14 of human EZH2.
Application
Anti-EZH2 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP)[1] and immunoblotting[2][3]
Anti-EZH2 (N-terminal) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:1,000 using K562 cell lysates and NIH3T3 cell lysates.
Biochem/physiol Actions
Enhancer of zeste homologue 2 (EZH2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. It is overexpressed in many cancers and is involved in malignant cell proliferation and invasion. EZH2 may be associated with senescence and apoptosis in gastric cancer. It may act as a valuable biomarker for the diagnosis and prognosis of gastric cancer. EZH2 may have function in regulation of gene transcription and chromatin structure.
Mutations in the EZH2 gene is implicated in the congenital disorder, Weaver syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Hanwen Zhang et al.
Oncogene, 39(5), 1041-1048 (2019-10-05)
Medulloblastoma (MB) is a malignant pediatric brain tumor for which new therapies are urgently needed. We demonstrate that treatment with EPZ-6438 (Tazemetostat), an enhancer of zeste homolog 2 (EZH2) inhibitor approved for clinical trials, blocks MB cell growth in vitro
Ana S A Cohen et al.
Human mutation, 37(3), 301-307 (2015-12-24)
Weaver syndrome (WS) is a rare congenital disorder characterized by generalized overgrowth, macrocephaly, specific facial features, accelerated bone age, intellectual disability, and susceptibility to cancers. De novo mutations in the enhancer of zeste homolog 2 (EZH2) have been shown to
Gorica Nikoloski et al.
Nature genetics, 42(8), 665-667 (2010-07-06)
In myelodysplastic syndromes (MDS), deletions of chromosome 7 or 7q are common and correlate with a poor prognosis. The relevant genes on chromosome 7 are unknown. We report here that EZH2, located at 7q36.1, is frequently targeted in MDS. Analysis
Lin Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35(7), 6649-6656 (2014-04-08)
The present meta-analysis aggregated the results of relevant studies to identify the role of zeste homolog 2 (EZH2) expression in gastric carcinogenesis among Asians. Related articles were found by searching the following electronic databases without language restrictions: PubMed, SpringerLink, Karger
H Chen et al.
Genomics, 38(1), 30-37 (1996-11-15)
To identify genes that map on human chromosome 21 (HC21) and that may contribute to the phenotype of Down syndrome (DS), exon trapping was applied to cosmid DNA from an HC21-specific library LL21NCO2-Q. More than 550 potential exons were cloned
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
