E6906

Anti-EZH2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-ENX-1, Anti-Enhancer of zeste homolog 2 (Drosophila)
• MDL number: MFCD09753855
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 100 kDa
• species reactivity: human
• technique(s): indirect immunofluorescence: 1:500-1:1,000 using HEK-293T cells fixed with paraformaldehyde-triton.; western blot: 1:500-1:1,000 using K562 cell lysates
• UniProt accession no.: Q15910
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... EZH2(2146)

Description

Immunogen

synthetic peptide corresponding to amino acids 477-493 of human EZH2, conjugated to KLH via a C-terminal added cysteine residue.

Application

Anti-EZH2 antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:500-1:1,000 using HEK-293T cells fixed with paraformaldehyde-triton and for western blotting at a dilution of 1:500-1:1,000 using K562 cell lysates.

Biochem/physiol Actions

Histone-lysine N-methyltransferase EZH2 is an enzyme encoded by the EZH2 gene in humans. It belongs to the multiprotein polycomb repressive complex 2.[1] Enhancer of zeste homologue 2 (EZH2) is crucially involved in epigenetic silencing by acting as a histone methyltransferase. It is overexpressed in many cancers and is involved in malignant cell proliferation and invasion. EZH2 may be associated with senescence and apoptosis in gastric cancer.[2] It may act as a valuable biomarker for the diagnosis and prognosis of gastric cancer.[3] EZH2 may have function in regulation of gene transcription and chromatin structure.[4]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)