Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

E4159

Sigma-Aldrich

Anti-EEF1D (126-140) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-EF-1D, Anti-EF1D, Anti-Elongation factor 1δ, Anti-FP1047

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~31 kDa

species reactivity

human

technique(s)

western blot: 1:500-1:2,000

UniProt accession no.

P29692

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... EEF1D(1936)

Immunogen

synthetic peptide corresponding to amino acids 126-140 of human EEF1D

Application

Anti-EEF1D (126-140) antibody produced in rabbit is suitable for western blotting at a dilution of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Elongation factor 1-δ is a protein encoded by EEF1D gene in humans. It is a subunit of EF-1 (strongly conserved in its C-terminal domain in humans) that regulates the elongation step of mRNA translation. The homology between the N-terminal domains of EF-1 δ proteins is low and is limited to the leucine zipper motif. EEF1D plays a key role in mediating BMP-6-induced transcriptional activation of E-cadherin in breast cancer cells. It is correlated with oncogenic transformation. EF-1 δ mRNA expression is significantly higher in cancerous than noncancerous oesophageal tissues. Over expression of EF-1 δ mRNA is related with lymph node metastases, advanced disease stages, poorer prognosis for patients with oesophageal carcinoma and malignant transformation of 16HBE cells induced by CdC12.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Not finding the right product?  

Try our Product Selector Tool.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
129K2730

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J Sanders et al.
Biochimica et biophysica acta, 1174(1), 87-90 (1993-07-18)
Copy-DNA clones containing the complete coding region of the human elongation factor-1 delta (EF-1 delta) mRNA have been isolated and characterized. We present the deduced amino acid sequence and observe in it a leucine zipper motif seen recently in EF-1
Yi-Xiong Lei et al.
Biomedical and environmental sciences : BES, 23(2), 151-157 (2010-06-03)
To study the alternative expression and sequence of human elongation factor-1 delta (human EF-1 delta p31) during malignant transformation of human bronchial epithelial cells induced by cadmium chloride (CdC12) and its possible mechanism. Total RNA was isolated at different stages
K Ogawa et al.
British journal of cancer, 91(2), 282-286 (2004-06-17)
Elongation factor-1 (EF-1) delta is a subunit of EF-1, which is a protein complex that participates in the elongation step of mRNA translation and has recently been considered to correlate with oncogenic transformation. However, there has been no information regarding

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service