E3779
Anti-Endostatin antibody produced in goat
affinity isolated antibody, lyophilized powder
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About This Item
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
mouse
technique(s)
immunohistochemistry (frozen sections): 15 μg/mL
indirect ELISA: 0.5-1.0 μg/mL
western blot: 0.1-0.2 μg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Col18a1(12822)
Immunogen
purified recombinant mouse endostatin expressed in Sf21 cells.
Application
Anti-Endostatin antibody produced in goat is suitable for the following applications:
- Immunohistochemistry (frozen sections) at a concentration of 15μg/mL
- Indirect ELISA at a concentration of 0.5-1.0μg/mL
- Western blotting at a concentration of 0.1-0.2μg/mL
Biochem/physiol Actions
Collagen α-1(XVIII) chain is a protein encoded by the COL18A1 gene in humans. It is mapped to chromosome 21q22.3. Endostatin is a 20kDA C-terminal fragment of collagen XVIII that is found in proteoglycan/collagen vessel walls and basement membranes. It helps in inhibiting angiogenesis, tumor growth and also attenuates endothelial cell migration. Endostatin treatment reduces the number of functional blood vessels and the matrix density in the granulation tissue. It is associated with inhibition of chorioallantoic membrane angiogenesis induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF).
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Johan Dixelius et al.
Cancer research, 62(7), 1944-1947 (2002-04-04)
Endostatin, an endogenous angiogenesis inhibitor, attenuates endothelial cell migration through an unknown mechanism. We show that endostatin induced tyrosine phosphorylation of focal adhesion kinase and paxillin, and promoted formation of focal adhesions and actin stress fibers, similar to fibroblast growth
T Sasaki et al.
Journal of molecular biology, 301(5), 1179-1190 (2000-09-01)
Endostatin is a fragment of the C-terminal domain NC1 of collagen XVIII that inhibits angiogenesis and tumor growth. We report the characterization of a collagen XV endostatin analogue and its parent NC1 domain, obtained by recombinant expression in mammalian cells.
U K Zatterstrom et al.
Cell structure and function, 25(2), 97-101 (2000-07-08)
The angiogenesis inhibitor endostatin is a 20 kDA C-terminal fragment of collagen XVIII, a proteoglycan/collagen found in vessel walls and basement membranes. The endostatin fragment was originally identified in conditioned media from a murine endothelial tumor cell line. Endostatin inhibits
W Bloch et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(15), 2373-2376 (2000-10-12)
Endostatin is a cleavage product of collagen XVIII that strongly inhibits tumor angiogenesis. To determine if endostatin affects other angiogenic processes, we generated full-thickness excisional wounds on the back of mice that were systemically treated with recombinant murine endostatin. No
S P Oh et al.
Genomics, 19(3), 494-499 (1994-02-01)
Types XV and XVIII collagen belong to a unique and novel subclass of the collagen superfamily for which we have proposed the name the MULTIPLEXIN family. Members of this class contain polypeptides with multiple triple-helical domains separated and flanked by
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