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E3762

Sigma-Aldrich

pFLAG-CMV™−5a,b,c Expression Vectors

0.5 mg/mL

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About This Item

MDL number:
MFCD03100886
UNSPSC Code:
12352200

tag

FLAG® tagged

grade

for molecular biology

form

buffered aqueous solution

concentration

0.5 mg/mL

shipped in

dry ice

storage temp.

−20°C

General description

p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.

Vector Maps and Sequences

Components

  • p-FLAG-CMV-5a Expression Vector (20μg)
  • p-FLAG-CMV-5b Expression Vector (20 μg)
  • p-FLAG-CMV-5c Expression Vector (20 μg)
  • pFLAG-CMV-5a-BAP control plasmid (20 μg)

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

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Hiroshi Tanaka et al.
Molecular cancer research : MCR, 7(4), 557-569 (2009-04-18)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily and has selective antitumor activity. Although TNF-alpha-induced intracellular signaling pathways have been well studied, TRAIL signaling is not fully understood. Here, we identified a novel TRAIL
Katherine L Bates et al.
Genetics, 178(4), 1989-2002 (2008-04-24)
High baselines of transcription factor activities represent fundamental obstacles to regulated signaling. Here we show that in Drosophila, quenching of basal activator protein 1 (AP-1) transcription factor activity serves as a prerequisite to its tight spatial and temporal control by
Nadine Pollak et al.
The Journal of biological chemistry, 282(46), 33562-33571 (2007-09-15)
NAD kinases (NADKs) are vital, as they generate the cellular NADP pool. As opposed to three compartment-specific isoforms in plants and yeast, only a single NADK has been identified in mammals whose cytoplasmic localization we established by immunocytochemistry. To understand
Jinlong Wang et al.
Biochimica et biophysica acta. Gene regulatory mechanisms (2018-07-18)
KRAB-containing zinc finger proteins (KZNF) constitute the largest family of transcriptional regulators in humans and play critical roles in normal development and tumorigenesis. However, the function and mechanism of most KZNFs remain unclear. Here, we report that ZNF496, a KZNF

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