E1783

Monoclonal Anti-eIF5A antibody produced in mouse

~1.0 mg/mL, clone IF5-88, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Eukaryotic translation initiation factor 5A, Anti-eIF-4D, Anti-eIF-5A-1, Anti-eIF-5A1
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: IF5-88, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~17 kDa
• species reactivity: canine, monkey, mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; western blot: 1-2 μg/mL using 3T3 total cell extract
• isotype: IgG1
• UniProt accession no.: P63241
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EIF5A(1984) ; mouse ... Eif5a(276770) ; rat ... Eif5a(287444)

Description

Application

Monoclonal Anti-eIF5A antibody produced in mouse is suitable for immunocytochemistry and western blot analysis at a working concentration of 1-2μg/mL using 3T3 total cell extract.

Biochem/physiol Actions

EIF5A (Eukaryotic translation initiation factor 5A-1) gene encodes a mRNA-binding protein involved in translation elongation. It also has an important role in mRNA turnover, mRNA decay, actin dynamics and cell cycle progression. It is also implicated in cell proliferation, synthesis of proteins involved in cell cycle control, and transport of viral mRNAs from nucleus to cytoplasm in association with Rev HIV-I protein. Higher eIF5A protein expression levels are present in lung adenocarcinomas tumors, suggesting eIF5A as a prognostic marker in lung carcinomas. It is a highly conserved protein that undergoes a unique and essential post-translational modification dependent on the polyamine spermidine, called hypusination. Reduction of eIF5A content is implicated in brain aging.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)