Skip to Content
MilliporeSigma
All Photos(1)

Documents

E1523

Sigma-Aldrich

Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD01633332
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 97 kDa

species reactivity

mouse, human

technique(s)

microarray: suitable
western blot: 1:8,000 using NIH 3T3 mouse fibroblasts cell extract

UniProt accession no.

Q13164

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPK7(5598)
mouse ... Mapk7(23939)

General description

Extracellular signal-regulated kinase 5 (ERK5), also referred to as big-MAP kinase 1 (BMK1), is a protein that belongs to mitogen-activated protein kinases family and is expressed mainly in skeletal muscle, kidney, heart and placenta. ERK5 contains a distinct 400-amino acid C-terminal and loop-12 domain, which includes two proline-rich regions.

Immunogen

synthetic peptide corresponding to amino acids 789-802 of human ERK5.

Application

Anti-ERK5 (big-MAPK, BMK1) an0tibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Extracellular signal-regulated kinase 5 (ERK5) has a critical role in different cellular processes like differentiation, proliferation and survival. Anti-ERK5 (big-MAPK, BMK1) antibody can be used to study the differential tissue expression and intracellular localization of the MAP Kinase isoform in normal and neoplastic tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

An evolutionarily conserved pathway controls proteasome homeostasis
Rousseau A and Bertolotti A
Nature, 536(7615), 184-184 (2016)
Extracellular signal-regulated kinase 7 (ERK7), a novel ERK with a C-terminal domain that regulates its activity, its cellular localization, and cell growth
Abe MK, et al.
Molecular and Cellular Biology, 19(2), 1301-1312 (1999)
Rajinder S Sawhney et al.
Journal of cellular physiology, 219(1), 152-161 (2008-12-18)
In metastatic cancer, high expression levels of vitronectin (VN) receptors (integrins), FAK, and ERK5 are reported. We hypothesized that integrin-mediated ERK5 activation via FAK may play a pivotal role in cell adhesion, motility, and metastasis. ERK5 and FAK phosphorylation when
Y Kato et al.
Nature, 395(6703), 713-716 (1998-10-28)
Epidermal growth factor (EGF) induces cell proliferation in a variety of cell types by binding to a prototype transmembrane tyrosine kinase receptor. Ligation of this receptor by EGF activates Erk1 and Erk2, members of the mitogen-activated protein (MAP) kinase family
Adrien Rousseau et al.
Nature, 536(7615), 184-189 (2016-07-28)
The proteasome is essential for the selective degradation of most cellular proteins, but how cells maintain adequate amounts of proteasome is unclear. Here we show that there is an evolutionarily conserved signalling pathway controlling proteasome homeostasis. Central to this pathway
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service