E0653

Ephrin-B1/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): Cek5-L, EFL-3, ELK-L, LERK-2, STRA-1
• MDL number: MFCD01867532
• UNSPSC Code: 51111800
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in NSO cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.01-0.5 ng/mL
• mol wt: monomer calculated mol wt 49.2 kDa; ~60 kDa by SDS-PAGE (reducing)
• packaging: pkg of 200 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: P52795
• storage temp.: −20°C
• Gene Information: mouse ... Efnb1(13641)

Description

General description

Extracellular domain of mouse ephrin-B1 (amino acids 1-229) is fused to the C-terminal 6X histidine-tagged Fc region of human IgG1.

Biochem/physiol Actions

Member of the ephrin ligand family that binds Eph receptor tyrosine kinases (EphA3, EphB1, EphB2, EphB3, and EphB4). Involved in pattern formation and morphogenesis. Ephrin-B1 is involved in integrin-mediated endothelial cell migration and attachment.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse EphB3/Fc in ELISA.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)