E0526

Endonuclease III from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, buffered aqueous glycerol solution, ≥10,000 units/mg protein

• Synonym(s): Nth protein, Endo III
• MDL number: MFCD02265700
• UNSPSC Code: 12352204

Properties

• biological source: Escherichia coli
• Quality Level: 200
• recombinant: expressed in E. coli overproducing strain
• assay: ≥90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥10,000 units/mg protein
• mol wt: ≥23 kDa (211 amino acids)
• technique(s): activity assay: suitable
• UniProt accession no.: P0AB83
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... nth(947122)

Description

Biochem/physiol Actions

Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme.

Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme. It possesses DNA glycosylase activity with broad substrate specificity for mutated pyrimidine derivatives especially thymine glycol (Tg) but also for 5-hydroxycytosine and 5,6-dihydrothymine. In addition, it has an apurinic/apyrimidinic (AP) lyase activity which cleaves the DNA backbone by β elimination. Endo III protects E. coli cells from radiation damage caused by x-ray and UV irradiation and its activity overlaps the activity of endonuclease VIII. When overexpressed, it can also protect E.coli cells from alkylation defects caused by methylmethanesulphonate.

Unit Definition

One unit will cleave 50% of 0.5 pmol double strand oligonucleotide substrate containing 5,6-dihydrothymine in 15 min. at 30 °C.

Physical form

Solution in 20 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.

Preparation Note

Purified in an iron bound form that confers the protein a light green color.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)