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technique(s)
proximity ligation assay: suitable
storage temp.
20-25°C
Application
Experiments conducted using Duolink In Situ reagents can detect and visualize protein interactions, protein expression levels and post translational modifications at the single molecule level in fixed cells and tissue samples.
To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA® probes, one PLUS and one MINUS, your choice of Detection Reagents. Recommended reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using Duolink ImageTool.
Duolink ImageTool is a dedicated and user-friendly software, specifically designed for objective quantification/counting of PLA signals in images generated from fluorescence microscopy. Both cells and tissue images may be analyzed. The nuclei are automatically detected and cytoplasm size estimated, enabling single cell statistical analysis of expression levels in tissue or cell populations. Furthermore regions of interest can be defined, a feature of particular relevance when studying tissue samples. Raw imaging data can be imported directly from the four major microscope vendors (Olympus, Leica, Nikon and Zeiss) as well as tiff and jpg. The results data can easily be exported to Microsoft Excel for further evaluation.
Demo version
When running the demo version you can import your own images and use all features of the software except getting the report of the analysis. To run the software in full mode you need a USB flash drive that works as a software protection key.
Download Demo Version
Duolink ImageTool Tutorial
Duolink ImageTool User Manual
Duolink ImageTool Test Images
Find answers to commonly asked question on our Duolink FAQ page
To perform a complete Duolink In Situ experiment you will need two primary antibodies (IHC or ICC/IF validated) that recognize two target epitopes. Additional reagents required include a pair of PLA® probes, one PLUS and one MINUS, your choice of Detection Reagents. Recommended reagents include Wash Buffers and Mounting Medium.
Analysis is carried out using standard immunofluorescence assay equipment. HRP/Novared is also available for bright field detection. Quick and reliable quantification can be performed using Duolink ImageTool.
Duolink ImageTool is a dedicated and user-friendly software, specifically designed for objective quantification/counting of PLA signals in images generated from fluorescence microscopy. Both cells and tissue images may be analyzed. The nuclei are automatically detected and cytoplasm size estimated, enabling single cell statistical analysis of expression levels in tissue or cell populations. Furthermore regions of interest can be defined, a feature of particular relevance when studying tissue samples. Raw imaging data can be imported directly from the four major microscope vendors (Olympus, Leica, Nikon and Zeiss) as well as tiff and jpg. The results data can easily be exported to Microsoft Excel for further evaluation.
Demo version
When running the demo version you can import your own images and use all features of the software except getting the report of the analysis. To run the software in full mode you need a USB flash drive that works as a software protection key.
Download Demo Version
Duolink ImageTool Tutorial
Duolink ImageTool User Manual
Duolink ImageTool Test Images
Find answers to commonly asked question on our Duolink FAQ page
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
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Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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