DOPA1003
Dopaminergic neurons, PARK7 (-/-) KO Line
IPSC derived
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About This Item
UNSPSC Code:
12352200
biological source
human
growth mode
Adherent
karyotype
2n=46
morphology
elongated/polarized, neuronal
relevant disease(s)
Parkinson′s disease
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
biallelic knock-out of PARK7 gene
Application
These products provide unique and versatile tools for the study of central nervous system (CNS) disorders, such as Alzheimer′s disease (AD), Parkinson′s disease (PD), autism, schizophrenia, and amyotrophic lateral sclerosis (ALS), as well as for drug screening and toxicology applications in the neural space.
Features and Benefits
This product contains cryo-preserved, pre-differentiated dopaminergic neurons derived from a footprint-free, karyotype normal human iPSC line with a homozygous knockout in PARK7 made using zinc fingers. It is designed for customers to generate mature dopaminergic neurons using the optimized maturation medium and supplements (DOPA1050, DOPA1051 sold separately). Mature and functional dopaminergic neurons can be obtained within 12-14 days. The dopaminergic precursors can be seeded on various culture vessel formats including 96-well plates on either glass or plastic surfaces and cultured as adherent cells. Shortly after seeding, the cells proliferate slightly for up to 4 days and show extensive neurite outgrowth and proper neuronal morphology. In general, at 12-14 days post-seeding, the cell population will contain >80% neurons, >30% TH+ dopaminergic neurons and < 15% GFAP+ astrocytes.
Culture Medium
User must also purchase Dopa Maturation Medium, DOPA1050 (50mL) or DOPA1051 (250mL). Sold separately.
Subculture Routine
No subculture routine is necessary. See technical bulletin for additional details.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Andrzej Swistowski et al.
Stem cells (Dayton, Ohio), 28(10), 1893-1904 (2010-08-18)
Human induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells represent a promising unlimited cell source for generating patient-specific cells for biomedical research and personalized medicine. As a first step, critical to clinical applications, we attempted to develop defined culture
Yiping Yan et al.
Stem cells translational medicine, 2(11), 862-870 (2013-10-12)
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, are unique cell sources for disease modeling, drug discovery screens, and cell therapy applications. The first step in producing neural lineages from hPSCs is
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