D9191

JumpStart^™ Taq ReadyMix^™ with dUTP

• UNSPSC Code: 41106300

Properties

• concentration: 2.5 units/reaction (50 μL reaction volume)
• shipped in: wet ice
• storage temp.: −20°C

Description

Features and Benefits

• The ideal ReadyMix for high throughput, quantitative PCR applications where carryover contamination is a concern
• The hot start mechanism using JumpStart Taq antibody prevents non-specific product formation; assembled PCR reactions can remain at room temperature for up to 2 hours without compromising performance
• The incorporation of dUTP provides the ability to eliminate contaminating PCR products from earlier reactions with uracil-DNA glycosylase; prevents carryover contamination

Other Notes

JumpStart^™ Taq ReadyMix^™ with dUTP for Quantitative PCR Kit provides the performance enhancements of our JumpStart Taq Antibody for hot start in a convenient, easy-to-use reaction mixture. The mix has no added dyes, making it ideal for performing high throughput quantitative PCR (qPCR) methods that rely on a fluorescent probe.

Principle

The ReadyMix has incorporated dUTP in place of TTP to facilitate carryover prevention. This nucleotide mixture provides the ability to eliminate contaminating PCR products by use of a Uracil-DNA glycosylase (UNG). The ready-to-use mixture of JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate. Simply add 25 μL of the 2× mix to the DNA template, primers, fluorescent probe, UNG, and water. At room temperature, the JumpStart Taq antibody inactivates the DNA polymerase. However, during the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• R4526 Reference Dye for Quantitative PCR, 100 ×, solution
• M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR