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D8259

Sigma-Aldrich

Deoxyribonucleic acid from Micrococcus luteus (lysodeikticus)

Type XI, Highly polymerized

Synonym(s):

DNA

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About This Item

CAS Number:
9007-49-2
MDL number:
MFCD00130921
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

type

Type XI

form

solid

storage temp.

2-8°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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Application

DNAs are useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols. Micrococcus luteus DNA contains a high G-C content (72%) versus other DNAs like Clostridium perfringens DNA, G + C (27%), making it useful in studies that compare the effects of base composition on DNA properties.
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.

Quality

Contains 2-4% protein

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Hasan Yardimci et al.
Nature structural & molecular biology, 21(1), 20-25 (2014-01-07)
In G1, two copies of the MCM2-7 helicase are recruited to each origin of replication. Whereas recruitment of the first MCM2-7 is likely to be analogous to the loading of sliding clamps around DNA, how the second MCM2-7 complex is
Garrett Hellenthal et al.
Science (New York, N.Y.), 343(6172), 747-751 (2014-02-18)
Modern genetic data combined with appropriate statistical methods have the potential to contribute substantially to our understanding of human history. We have developed an approach that exploits the genomic structure of admixed populations to date and characterize historical mixture events
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