D5444

Anti-DCP1A (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DCP1 Decapping enzyme 1, homolog A, Anti-SMAD4IP1, Anti-SMIF, Anti-Smad 4-interacting transcription factor, Anti-Smad 4-interacting transcriptional co-activator
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: mouse, human, rat (predicted)
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using cell lystes of HEK-293T; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells; western blot: 1-2 μg/mL using cell lysates of HEK-293T
• UniProt accession no.: Q9NPI6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DCP1A(55802) ; mouse ... Dcp1a(75901) ; rat ... Dcp1a(361109)

Description

General description

Dcp1 colocalizes with Dcp2 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. Anti-DCP1A (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at C-terminal of human DCP1A conjugated to KLH. Two distinct genes of human DCP1 were identified, DCP1A and DCP1B, which share ~70% homology in their N-terminal and ~30% homology in their full length.

Application

Anti-DCP1A antibody produced in rabbit is suitable for immunoprecipitation at a working concentration of 5-10 μg using cell lystes of HEK-293T, indirect immunofluorescence at 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells and western blot analysis at 1-2 μg/mL working concentration using cell lysates of HEK-293T. It was used as a primary antibody at a working dilution of 1:200 in the immunofluorescence experiment of HeLa cells treated with 5-fluorouracil to study the assembly of stress granules based on RNA incorporation.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence-cell culture cells (1 paper)

Biochem/physiol Actions

Dcp1 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp2 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)