D5167
Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse
clone 70.1, ascites fluid
Synonym(s):
Anti-CILD1, Anti-DIC1, Anti-ICS1, Anti-PCD
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About This Item
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
70.1, monoclonal
contains
15 mM sodium azide
species reactivity
marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog
technique(s)
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:2,000 using a chick brain extract
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DNAI1(27019) , DNAI2(64446)
rat ... Dnai2(360654)
General description
Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7).
Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region.
Immunogen
cytoplasmic dynein from chicken embryo brain
Application
Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in:
- perturbation assays
- western blotting
- immunofluorescence microscopy
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunocytochemistry
- immunopurification
Biochem/physiol Actions
Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kinesin, Dynein, and Intracellular Transport.
Lodish H, Berk A, Zipursky SL, et al
Molecular and Cellular Biology, 4, Section 19-Section 19 (2000)
Jonne A Raaijmakers et al.
The EMBO journal, 31(21), 4179-4190 (2012-10-05)
The microtubule motor protein kinesin-5 (Eg5) provides an outward force on centrosomes, which drives bipolar spindle assembly. Acute inhibition of Eg5 blocks centrosome separation and causes mitotic arrest in human cells, making Eg5 an attractive target for anti-cancer therapy. Using
Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome
Casenghi M, et al.
Journal of Cell Science, 118(21), 5101-5108 (2005)
Eliza Zyłkiewicz et al.
The Journal of cell biology, 192(3), 433-445 (2011-02-02)
Ndel1 has been implicated in a variety of dynein-related processes, but its specific function is unclear. Here we describe an experimental approach to evaluate a role of Ndel1 in dynein-dependent microtubule self-organization using Ran-mediated asters in meiotic Xenopus egg extracts.
Nucleoporin translocated promoter region (Tpr) associates with dynein complex, preventing chromosome lagging formation during mitosis
Nakano H, et al.
The Journal of Biological Chemistry, 285(14), 10841-10849 (2010)
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