D3316

Anti-DEDAF antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DED Associated Factor, Anti-Death Effector Domain-Associated Factor
• MDL number: MFCD03791670
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~32 kDa
• species reactivity: human, rat, mouse
• concentration: ~0.5 mg/mL
• technique(s): microarray: suitable; western blot: 0.5-1 μg/mL
• UniProt accession no.: Q8N488
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... RYBP(23429) ; mouse ... Rybp(56353) ; rat ... Rybp(312603)

Description

General description

DEDAF is identical to the transcriptional repressor RYBP (RING1 and YY1 binding protein). DEDAF/RYBP is expressed in multiple tissues and cell lines.

Immunogen

synthetic peptide corresponding to amino acids 215-228 of human DEDAF.

Application

Anti-DEDAF antibody produced in rabbit is suitable for microarray and western blot analysis at a working concentration of 0.5-1μg/mL using human A549 alveolar epithelial), human HepG2 (hepatoblastoma), and mouse 3T3 (fibroblasts) cell lysates.

Biochem/physiol Actions

DEDAF is a protein that interacts with DED (death effector domain) of caspase-8 and -10 and FADD (Fas-associated death domain). With FADD, it augments the formation of CD95/FADD/caspase-8 complexes at the cell membrane. It interacts with DED-containing DNA binding protein (DEDD) in the nucleus indicating its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.