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D3160

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, male

Synonym(s):

DNA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

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General description

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Application

Deoxyribonucleic acid from human placenta is used as blocking agent in Southern hybridizations. It was used to compare the efficiency of DNA quantification methods.[1]
For use in Southern hybridizations.

Components

DNA is supplied in a solution of 10mM Tris-HCl (pH 8.4), with 1mM EDTA.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
Xin Cai et al.
Molecular cell, 54(2), 289-296 (2014-04-29)
The innate immune system deploys a variety of sensors to detect signs of infection. Nucleic acids represent a major class of pathogen signatures that can trigger robust immune responses. The presence of DNA in the cytoplasm of mammalian cells is
Chengzu Long et al.
Science (New York, N.Y.), 345(6201), 1184-1188 (2014-08-16)
Duchenne muscular dystrophy (DMD) is an inherited X-linked disease caused by mutations in the gene encoding dystrophin, a protein required for muscle fiber integrity. DMD is characterized by progressive muscle weakness and a shortened life span, and there is no
Sriram Kosuri et al.
Nature methods, 11(5), 499-507 (2014-05-02)
For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which

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