D2191
Monoclonal Anti-DC-SIGN1 antibody produced in mouse
clone 120507, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-CD209, Anti-Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
120507, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
flow cytometry: 2.5 μg using 106 cells
immunocytochemistry: 8-25 μg/mL
immunohistochemistry: 8-25 μg/mL using fixed cells and tissue sections
western blot: 1 μg/mL
isotype
IgG2b
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CD209(30835)
General description
DC-SIGN is a type II protein C-type lectin that binds mannose-rich molecules such as ICAM-3 and ICAM-2 in a calcium dependent manner. DC-SIGN, a 44 kDa molecule, is identical to the HIV-1 binding protein.
Specificity
Recognizes human DC-SIGN on transfected cells and on monocyte derived dentritic cells. The antibody does not react with either parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Immunogen
purified from NIH/3T3 cells transfected with recombinant human DC-SIGN.
Application
Monoclonal Anti-DC-SIGN1 antibody produced in mouse is suitable for flow cytometry, immunocytochemistry at a working concentration of 8-25 μg/mL, immunohistochemistry at 8-25 μg/mL using fixed cells and tissue sections and western blotting at 1 μg/mL.
Biochem/physiol Actions
DC-SIGN1 is involved in molecular interactions between dendritic cells and resting T-cells. DC-SIGN interacts with viruses such as X4, R5, X4R5 HIV-1 strains, HIV-2, and SIV. It captures HIV-1 through its interaction with the gp120 envelope glycoprotein and promotes efficient infection of CD4+ T cells that co-express chemokine receptors.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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I Tirado-González et al.
Placenta, 33(4), 257-263 (2012-01-21)
Normal pregnancy and spontaneous abortion in humans and mice are associated with immune responses. The decidua harbors dendritic cells identifiable in humans by their expression of DC-SIGN. Because dendritic cells are essential for immune response regulation, decidual DC-SIGN+ cells may
T B Geijtenbeek et al.
Cell, 100(5), 575-585 (2000-03-18)
Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of
B M Curtis et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(17), 8356-8360 (1992-09-01)
The binding of the human immunodeficiency virus (HIV) envelope glycoprotein gp120 to the cell surface receptor CD4 has been considered a primary determinant of viral tropism. A number of cell types, however, can be infected by the virus, or bind
T B Geijtenbeek et al.
Cell, 100(5), 587-597 (2000-03-18)
Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of
T B Geijtenbeek et al.
Nature immunology, 1(4), 353-357 (2001-03-23)
Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports
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