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D0572

SAFC

Dulbecco′s Modified Eagle′s Medium - high glucose

AQmedia™, With 4500 mg/L glucose, 25mM HEPES, sodium bicarbonate and L-Ala-L-Gln, HEPES modification, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, DMEM

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About This Item

UNSPSC Code:
12352207

sterility

sterile-filtered

product line

AQmedia™

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin tested

components

NaHCO3: 3.7 g/L
glucose: 4.5 g/L (Dextro)
L-glutamine: 0.584 g/L
phenol red: 0.0159 g/L
HEPES: 5.958 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBD2909
RNBC0257

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William Diehl-Jones et al.
Journal of pediatric gastroenterology and nutrition, 61(5), 583-590 (2015-10-28)
The aim of the present study was to determine the in vitro effect(s) of a bovine-based human breast milk fortifier (HMF) on human intestinal cells. HMF increases the expression of BCL2/adenovirus E1B 19 kDa protein-interacting protein (Bnip3) and cell death;
Carla Luana Dinardo et al.
PloS one, 10(12), e0145062-e0145062 (2015-12-15)
Recent evidence shows the rigidity of vascular smooth muscle cells (VSMC) contributes to vascular mechanics. Arterial rigidity is an independent cardiovascular risk factor whose associated modifications in VSMC viscoelasticity have never been investigated. This study's objective was to evaluate if
Elena Seiradake et al.
The EMBO journal, 30(21), 4479-4488 (2011-09-29)
Brain wiring depends on cells making highly localized and selective connections through surface protein-protein interactions, including those between NetrinGs and NetrinG ligands (NGLs). The NetrinGs are members of the structurally uncharacterized netrin family. We present a comprehensive crystallographic analysis comprising

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