D0572

Dulbecco′s Modified Eagle′s Medium - high glucose

AQmedia^™, With 4500 mg/L glucose, 25mM HEPES, sodium bicarbonate and L-Ala-L-Gln, HEPES modification, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): DME, DMEM
• UNSPSC Code: 12352207

Properties

• sterility: sterile-filtered
• product line: AQmedia^™
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin tested
• components: NaHCO₃: 3.7 g/L; glucose: 4.5 g/L (Dextro); L-glutamine: 0.584 g/L; phenol red: 0.0159 g/L; HEPES: 5.958 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable