D0319

Anti-Dorfin (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Double ring-finger protein, Anti-Protein p38 interacting with transcription factor Sp1, Anti-RING finger protein 19, Anti-RNF19, Anti-p38 protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~105 kDa
• species reactivity: rat (predicted), human, mouse
• concentration: ~1.5 mg/mL
• technique(s): western blot: 1-2 μg/mL using HEK-293T cells expressing human Dorfin
• UniProt accession no.: Q9NV58
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... RNF19A(25897) ; mouse ... Rnf19a(30945)

Description

General description

Dorfin is an E3 ubiquitin ligase that contains a RING finger/IBR (in between-RING-finger) domain at its N-terminus. Dorfin resides in the perinuclear region and forms aggresome-like structures, that contain misfolded ubiquitylated proteins.

Immunogen

synthetic peptide corresponding to amino acids 1-19 of human dorfin, conjugated to KLH. This sequence is identical in mouse dorfin and highly conserved (two amino acid substitutions) in rat dorfin.

Application

Anti-Dorfin (N-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2 μg/mL using HEK-293T cells expressing human Dorfin.

Biochem/physiol Actions

Dorfin mediates E3 ubiquitin ligase activity. It interacts with human E2s UbcH7 and UbcH8. The C-terminus of Dorfin can recognize mutant SOD1 proteins, which cause familial ALS (FALS). Dorfin binds and ubiquitinylates various SOD1 mutants derived from FALS and enhances their degradation, but has no effect on the stability of wild-type SOD1. Over expression of this protein protects neuronal cells against the toxic effect of mutant SOD1 and reduces SOD1 inclusions. It has been localized to aggresomes and ubiquitin positive inclusions in several neurodegenerative diseases including: PD, dementia with Lewy bodies, multiple system atrophy and ALS.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)