CS1050
UDP-Galactosyltransferase Assay Kit
sufficient for 100 tests
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About This Item
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 100 tests
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... B4GALT1(2683)
Application
The kit assay is based on the transfer of radioactively labeled galactose from UDP-galactose to N-acetyl-D-glucosamine by UDP-galactosyltransferase. The reaction product, a radiolabeled N-acetyl-D-lactosamine, is then purified by ion-exchange chromatography and quantified.
Biochem/physiol Actions
UDP-galactosyltransferase (β4GalT1) is a trans-Golgi membrane bound protein that participates in protein processing in the Golgi apparatus. UDP-galactosyl-transferase catalyzes the transfer of galactose from UDP-galactose to N-acetyl-D-glucosamine. The exclusive location of UDP-galactosyltransferase in the Golgi apparatus in the vast majority of tissues and cells makes it a convenient biochemical marker for Golgi membranes.
Analysis Note
The kit provides a convenient method for quantitative estimation of the UDP-galactosyltransferase activitity in cell or tissue extracts.
Kit Components Also Available Separately
Product No.
Description
SDS
- C2728Mini-Columns, capacity 2.4 mL, Overall H 1.5 in. 100 eaSDS
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