The centrifugation parameter is 200,000g, and the g units do not depend on the rotor size. The customer can use their rotor and centrifuge if the instrument (rotor and centrifuge) can achieve the required speed. It is important to use tubes that are stable for this condition and are appropriate for fraction collection.
CS0750
Caveolea/Rafts Isolation Kit
sufficient for 20 purifications
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 20 purifications
shipped in
dry ice
storage temp.
−20°C
Application
The kit provides an easy method for obtaining fractions enriched with caveolea/rafts proteins from tissue culture cells. This method for the isolation of caveolae and rafts is based on the insolubility of these structures in ice cold non-ionic detergent Triton X-100. The kit includes all reagents for cells lysis, density gradient, and the isolation of caveolea/rafts protein enriched fraction, as well as two markers for caveolea/rafts compartments, anti-caveolin-1 and cholera toxin B subunit-peroxidase (CTB-HRP).
Biochem/physiol Actions
Caveolae and lipid rafts are important cholesterol and sphingolipid-rich structures that represent a subcompartment or microdomain of the plasma membrane and are implicated in cellular transport and signal transduction related events.
Kit Components Only
Product No.
Description
- Lysis buffer 100 mL
- Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg
- Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL
- Anti-Caveolin-1 antibody produced in rabbit 50 μL
- Triton X-100 2 mL
- OptiPrep Density Gradient Medium 100 mL
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
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Can the centrifugation protocol for the CS0750 Caveolea/Rafts Isolation Kit be modified, given that it recommends setting up a gradient with 9ml Optiprep in 10ml tubes for ultracentrifugation at 200,000g? Considering that no rotor fits tubes of this size except for 50ml and 5ml rotors.
1 answer-
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