grade
Molecular Biology
for molecular biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.
The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.
Vector Maps and Sequences
The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.
Vector Maps and Sequences
Features and Benefits
- Fast and convenient 96 well plate format
- Cleaner analysis due to strongly bound antibody
- Rapid, sensitive, quantitative results
Components
The IP Vector Kit contains:
- pFLAG-CMV-2 Expression Vector (10 μg)
- pc-Myc-CMV-2 Expression Vector (10 μg)
- pFLAG-CMV-2-p53 control plasmid (20 μg)
- pc-Myc-CMV-2-Large T antigen control plasmid (10 μg)
- pc-Myc-CMV-2-BAP control plasmid (10 μg)
- Verification Primer-MF (2 nmol)
- Verification Primer-MR (2 nmol)
Kit Components Only
Product No.
Description
- pc-Myc-CMV-2 10 μg
- pc-Myc-CMV-2-BAP 10 μg
- pc-Myc-CMV-2-Large T antigen 10 μg
- pFLAG-CMV™-2 10 μg
- pFLAG-CMV™-2-p53 20 μg
- Verification Primer-MF 2 nmol
- Verification Primer-MR 2 nmol
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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A short polypeptide marker. Sequence useful for recombinant protein identification and purification.
Hopp, T. P., et al.
Biotechnology, 6, 1204-1210 (1988)
G I Evan et al.
Molecular and cellular biology, 5(12), 3610-3616 (1985-12-01)
Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also
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