LY9

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• packaging: tube of 5 μg 99061826-DNA-5UG; pkg of vial of cells 99061826-1VL
• ImmunoDon: (C57BL/6 x BALB/c)F1
• Myeloma: P3x63Ag8.653
• Reactivity: Anti-HLA DR7, DRw10,DRw53
• technique(s): cell culture | mammalian: suitable
• isotype: IgG3
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse x Mouse hybridoma

Cell Line Description

LY9 was produced from a (C57BL/6 x BALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid B-cell line Leakar and B. pertussis adjuvant. The genotype of the B-cell donor for this line was HLA-A2,B13, Cw- DR7, DQ 2/A24,B13, Cw-,DR7 B35, Cw4, DR3, DQ2. Mice spleen was removed 3 days later and fused with the myeloma P3x63Ag8.653. LY9 monoclonal antibodies were shown to react with cells expressing DRw53, DR7 (in the absence of DRw53) and DRw10. LY9 is also known as LK261.

Immunogen

B cell line Leakar HLA A 2,24; B13,35;C4; DR 7,3

Application

Immunofluorescence, cytotoxicity

Culture Medium

RPMI 1640 + 2mM glutamine + 1 mM NaP + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.